ライブイメージャー開発とエクソソーム測定
- Develpment of Live Imagers and Measurement of Exosomes -
我々の体は様々な細胞から構成され、それら細胞は種類ごとに異なる機能を有しております。一方、同じ種類の細胞であっても、細胞が配置された場所、老化度合い、病的状態によって、個々の細胞が発揮する能力に差が出るのみならず、機能そのものも変化します。これら細胞機能の質と量の変化は、細胞内のエネルギー代謝と密接に関わっており、エネルギー産生・消費機構を評価することは生体・細胞の状態を測定する新たな技術開発に繋がります。当研究室では、生体レベルと細胞レベルの双方でエネルギー代謝を解明することで、創薬標的の探索やスクリーニング技術の開発を行います。
Our bodies are composed of numerous cells with diverse functions. Even if the cells share their fates, cellular functions in individual cells differ, depending on their locations within the body, aging, and pathological status, etc. These differences in cellular functions are closely related to cellular energy metabolism. Elucidation of the mechanisms underlying energy production and consumption could lead to the development of tools for drug discovery. To establish new methods, we are studying energy metabolism both in the whole body as well as individual cells.
乳酸菌からの細胞外小胞上のSLAPというタンパク質は、我々の免疫系に作用し悪玉菌を積極的に除去する作用を有します。また、乳酸菌長期醗酵でもEVsは残存しており、多くの乳酸菌ペプチドや機能性脂質が集積しております。これらは、マウスの体重増加抑制にも繋がります。
Extracellular vesicles derived from lactic acid bacteria carry a protein called SLAP on their surface, which interacts with our immune system and promotes the active elimination of harmful bacteria. In addition, EVs remain present even after long-term fermentation of lactic acid bacteria, and they accumulate various lactic acid bacterial peptides and functional lipids.This also contributes to the suppression of body weight gain in mice.
Surface Layer-Associated Protein on Nanoparticles from Lactobacillus helveticus Enhances the Response to LPS in RAW264.7 Cells. Yamamoto C. Fujiwara N.Sholihah AI Kizaki R.Ito D.Yokoi C.Furukawa S.Yamamoto M.Koyama H.Hirata Y.Furuta K.Takemori H.Current Microbiology 2026 83: 201
Kotomi Chikama, Koutarou Terada, Chika Yamamoto, Mana Yamamoto, Ayano Hojo, Kotaro Fujioka, Hideto Torii, Lee Wah Lim, Hiroshi Takemori. Journal of Food Science 90 e70518
Extracellular Vesicle-Like Nanoparticles, Artificially Created by Heat Treatment of Euglena gracilis, Exhibit Autofluorescence and Suppress IL-6 Expression in RAW264.7 Cells
Ayumi Sakurai, Kyosuke Egashira, Rinka Kizaki, Ika Adhani Sholihah, Hiroko Koyama, Shigeo Takashima, Yuji O. Kamatari, Irmanida Batubara, Dyah Iswantini, Christofora Hanny Wijaya, Ika D Ana, Anggtaini Barlian, Yoko Hirata, Kyoji Furuta, Hiroshi Takemori BioNanoScience 2025 15(2) 271
GIF-2250は細胞内のエンドソームを染色しますが、細胞外では
エクソソームを染色できます。緑がエクソソームです。赤もエクソ
ソームの膜染色です。これらは、100nmの極微小小胞です。
エクソソームのような細胞外小胞のことをＥＶと呼びます。
Simple methods for measuring milk exosomes using fluorescent
Saho Furukawa, Kyoka Kawaguchi, Kotomi Chikama, Ryohei Yamada,
Yuji O. Kamatari, Lee Wah Lim, Hiroko Koyama, Yasuo Inoshima,
Mitsushi J. Ikemoto, Saishi Yoshida, Yoko Hirata,Kyoji Furuta,
Hiroshi Takemori
BBRC 2024: 149505
新たなミトコンドリアラベル化剤(MitoMM)を開発しました。ライブイメージ・固定化後の抗体染色（界面活性化剤利用可）に利用できます。
We have developed new mitochondrial imagers (MitoMM) that are able to use in living cells and fixed cells even in the presence of detergents.
メラノサイトとケラチノサイトのうち、
ケラチノサイトのミトコンドリアをラベル
し、メラノソームの送達を可視化しました。
LysoKK(GIF-2259)とMitoMMでマイトファジーを検出できます。
GIF-2250（緑）は、メラノソーム（黒)の分解を評価できます。
Melanin-concentrating hormone receptor 1 is discarded by exosomes after internalization
Ryohei Yamada, Momoka Michimae, Akie Hamamoto, Hiroshi Takemori
Biochemical and Biophysical Research Communications 2024 710 139917
Intermittent inhibition of FYVE finger-containing phosphoinositide kinase induces melanosome degradation in B16F10 melanoma cells
Mol Bio Rep 2022 50: 5917-5930
Visualization of mitophagy using LysoKK, a 7-nitro-2,1,3- benzoxadiazole-(arylpropyl)benzylamine derivative
Takemori H, Koga K, Kawaguchi K, Furukawa S, Ito S, Imaishi J, Watanabe M, Maeda M, Mizoguchi M, Oh-Hashi K, Hirata Y, Furuta K
Mitochondrion 2022 62:176-180
The new live imagers MitoMM1/2 for mitochondrial visualization
Maeda M, Suzuki M, Takashima S, Sasaki T, Oh-Hashi K, Takemori H.
Biochem Biophys Res Commun 2021 562:50-54. doi: 10.1016/j.bbrc.2021.05.040.
Monitoring of Glutamate-Induced Excitotoxicity by Mitochondrial Oxygen Consumption.
Kumagai A, Sasaki T, Matsuoka K, Abe M, Tabata T, Itoh Y, Fuchino H, Wugangerile S, Suga M, Yamaguchi T, Kawahara H, Nagaoka Y, Kawabata K, Furue MK, Takemori H
Synapse. 2019 e22067
特願2021-012443 「化合物、細胞性小胞染色剤および細胞性小胞の蛍光染色方法」
竹森 洋、古田享史、森田洋子 出願人： 東海国立大学機構 2021/1/28
Salt Inducible Kinases Are Critical Determinants of Female Fertility
Armouti M, Winston N, Hatano O, Hobeika E, Hirshfeld-Cytron J, Liebermann J, Takemori H, Stocco C.
Endocrinology. 2020 Apr 28:bqaa069. doi: 10.1210/endocr/bqaa069.
Mouse Model of Metformin-Induced Diarrhea
Takemori h, Hamamoto A, Isogawa K Ito M, Takagi M Morino H, MIura T, Oshida K, Shibata T
BMJ Open Diabetes Res Care 2020 8: 1 DOI: 10.1136/bmjdrc-2019-000898
Hirata Y, Iwasaki T, Makimura Y, Okajima S, Oh-Hashi K, Takemori H.
Toxicology. 2019 418: 1-10
A Simple Method for Labeling Human Embryonic Stem Cells Destined to Lose Undifferentiated Potency.
Kumagai A, Suga M, Yanagihara K, Itoh Y, Takemori H, Furue MK.
Stem Cells Transl Med. 2016 5 :275-81
Sanosaka M, Fujimoto M, Ohkawara T, Nagatake T, Itoh Y, Kagawa M, Kumagai A, Fuchino H, Kunisawa J, Naka T, Takemori H.
Immunology. 2015 145:268-278.
Involvement of SIK3 in glucose and lipid homeostasis in mice.
Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, Takikawa H, Fukusato T, Koura M, Nish M, Hamsten A, Silveira A, Bertorello AM, Kitagawa K, Nagaoka Y, Kawahara H, Tomonaga T, Naka T, Ikegawa S, Tsumaki N, Matsuda J, Takemori H.
PLoS One. 2012 7: e37803
SIK2 is a key regulator for neuronal survival after ischemia via TORC1-CREB.
Sasaki T, Takemori H, Yagita Y, Terasaki Y, Uebi T, Horike N, Takagi H, Susumu T, Teraoka H, Kusano K, Hatano O, Oyama N, Sugiyama Y, Sakoda S, Kitagawa K.
Neuron. 2011 69:106-119
Silencing the constitutive active transcription factor CREB by the LKB1-SIK signaling cascade.
Katoh Y, Takemori H, Lin XZ, Tamura M, Muraoka M, Satoh T, Tsuchiya Y, Min L, Doi J, Miyauchi A, Witters LA, Nakamura H, Okamoto M.
FEBS J. 2006 273: 2730-2748
The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector.
Screaton RA, Conkright MD, Katoh Y, Best JL, Canettieri G, Jeffries S, Guzman E, Niessen S, Yates JR 3rd, Takemori H, Okamoto M, Montminy M.
Cell. 2004 119:61-74.
